HIV-1 PROVIRAL DNA QUANT

Empowering HIV management through Advances Proviral DNA testing

Welcome to Taqgen Diagnostic, a leading provider of HIV Proviral DNA Load Testing services. Our commitment to excellence and innovation in diagnostics ensures that individuals living with HIV receive accurate and actionable insights for effective disease management.

Why choose our HIV proviral DNA load testing services?

• Comprehensive Viral Load Assessment: Our state-of-the-art Proviral DNA Load Testing goes beyond traditional methods, offering a comprehensive assessment of the proviral DNA in the bloodstream for a more detailed understanding of viral activity.
• Early Detection and Treatment Optimization: Early detection of HIV is crucial for timely intervention. Our test not only aids in the initial diagnosis but also plays a pivotal role in optimizing treatment plans by providing continuous monitoring of proviral DNA levels.
• Personalized Treatment Strategies: We believe in the power of personalized medicine. Our detailed Proviral DNA Load results contribute to the development of customized treatment plans, allowing healthcare providers to tailor interventions based on individual patient needs.
• Resistance Analysis: Our testing includes a thorough analysis of potential resistance mutations, providing valuable information to guide healthcare professionals in choosing the most effective antiretroviral therapies.

How our Services Work

1. Sample Collection: Healthcare providers collect a blood sample from the patient through a simple and minimally invasive procedure.
2. Advanced Laboratory Analysis: Our certified laboratories utilize cutting-edge technology to analyze the proviral DNA load with a high level of sensitivity and precision.
3. Timely Results Delivery: Results are delivered promptly to healthcare professionals through secure channels, ensuring quick decision-making and intervention.

Benefits of choosing Taqgen

• Clinical Excellence: Our team of experienced professionals is dedicated to delivering accurate and clinically relevant results to support healthcare decision-making.
• Confidentiality and Privacy: We prioritize patient confidentiality, implementing stringent security measures to protect personal health information.
• Commitment to Quality: Taqgen adheres to the highest standards of quality assurance, ensuring the reliability and accuracy of our HIV Proviral DNA Load Testing services.

Empower your patients and healthcare providers with our advanced HIV Proviral DNA Load Testing services. Partner with Taqgen Diagnostics for cutting-edge diagnostics, personalized solutions, and a commitment to improving the lives of individuals affected by HIV. Contact us today to learn more.

Shipping Guidelines

1. Ship frozen plasma sample on dry ice only.
2. If shipment will be delayed for more than 24 hours, freeze plasma at -20°C (up to 30 days) until shipment on dry ice.

Sample Required

Collection Container/Tube: EDTA Tube, 3 mL Sample Type: Whole Blood Sample Volume: 1.5 to 2 mL whole blood Collection Instructions: 1. Blood collection with 21 gauge Syringes using BD vacutainer. 2. Aliquot whole blood into EDTA tube only.

Specimen Stability Information

Useful For • Detection and quantification of HIV-1 DNA in blood of patients with HIV infection (i.e., sero-positive) • Monitoring disease progression in chronic HIV infection • Monitoring response to long-term ARV treatment and its effect on HIV latency.

Test Details: Method: Quantitative Real-time PCR using Taqman technology. Specificity: Assay is designed with specific primers and Taqman probe for in vitro quantification of all the genetic subtypes of HIV-1 group M against the LTR gene. Target Selected: HIV-1 LTR, Highly conserved sequence among HIV-1 group M is selected for detection. Sample: EDTA Whole Blood. Limit of detection: 100 copies/ml. Measuring Range: 100 – 100 Million copies/ml. PCR system: CFX96™ Real-time PCR System-IVD (BIORAD). Software: Bio-Rad CFX Manager IVD Edition 1.6. Data Analysis Mode: PCR Quantification with standard curve.

Test Interpretation: HIV Proviral DNA Real-time PCR is done by using specific primers and sequence-specific Taqman Probe to conserved regions of HIV-1 Proviral DNA that exclude the HIV-2 infection. HIV-1 standard, Negative control, and Internal controls are run along with duplicates of patient samples to ensure more accurate and precise detection of Proviral HIV-1 DNA with least variation. “Detected” results indicate the presence of HIV-1 infection. Two consecutive positive HIV-1 proviral DNA test results are necessary for the Diagnosis of HIV-1 infection in infants <2 years of age also for Patients. “NOT Detected” indicates either the absence of HIV-1 Proviral DNA in the patient’s Specimen or HIV-1 Proviral DNA level is below the detection limit of this assay.

Clinical Significance: Proviral Human Immunodeficiency Virus type-1 (HIV-1) proviral DNA is increasingly used to measure the HIV-1 cellular reservoirs, a helpful marker to evaluate the efficacy of antiretroviral therapeutic regimens in HIV-1–infected individuals. Furthermore, the proviral DNA load represents a specific marker for the early diagnosis of perinatal HIV-1 infection and might be predictive of HIV-1 disease progression independently of plasma HIV-1 RNA levels and CD4+ T-cell counts. The quantification indicates either the cell-associated HIV DNA or HIV DNA+ cell or HIV Proviral Load. DNA levels were calculated and expressed as copies per milliliter of whole blood. HIV-1 DNA levels in whole Blood were measured at baseline (prior to initiation of study treatment) and at weeks 16, 32, 48, 64, 80, and 96. Note: The linear dsDNA molecule is the precursor to the provirus. HIV infection of target cells generates a number of nonintegrated DNA species. These unintegrated HIV-1 DNA forms, which are linear or circularized, arise when viral dsDNA fails to integrate into the host genome, or linear unintegrated HIV-1 DNA may form. Therefore, aside from proviral DNA (HIV-1 dsDNA integrated into the host genome), several unintegrated forms also persist intracellularly. It is worth mentioning that cell-associated HIV-1 DNA can be found in both actively and latently infected cells. The new technique was developed to quantify proviral HIV-1 and all other forms of HIV DNA present intracellularly using a TaqMan real-time PCR assay.

Limitation of Assay: The prime reason for paradoxical results is contamination during specimen collection, inappropriate selection of specimen, presence of inherent PCR inhibitors, loss, or degradation of DNA.

Day(s) Performed: Monday through Saturday

Report Available: 1 to 3 days

Test Code Information

Reference:
• DeSimone J, Pomerantz R. New methods for the detection of HIV. Clin Lab Med 22 (2002) 573–592.
• Beloukas A, Paraskevis D, Haida C, Sypsa V, Hatzakis A. Development and Assessment of a Multiplex Real-Time PCR Assay for Quantification of Human Immunodeficiency Virus Type 1 DNA. Journal of Clinical Microbiology. 2009;47(7):2194-2199.
• Oliveira M, Gianella S, Letendre S, Scheffler K, Kosakovsky Pond S, Smith D et al. Comparative Analysis of Cell-Associated HIV DNA Levels in Cerebrospinal Fluid and Peripheral Blood by Droplet Digital PCR. PLOS ONE. 2015;10(10):e0139510
• Saitoh A, Hsia K, Fenton T, Powell C, Christopherson C, Fletcher C et al. Persistence of Human Immunodeficiency Virus (HIV) Type 1 DNA in Peripheral Blood Despite Prolonged Suppression of Plasma HIV-1 RNA in Children. The Journal of Infectious Diseases. 2002;185(10):1409-1416.
• Desire N, Dehee A, Schneider V, Jacomet C, Goujon C, Girard P et al. Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real-Time PCR Assay. Journal of Clinical Microbiology. 2001;39(4):1303-1310.