TaqGen® Whole Blood Genomic DNA Purification
The Taqgen® Whole Blood Genomic DNA Purification, a spin column based kit, provide an easy, rapid and efficient purification of high quality genomic DNA from whole blood and body fluid. The purified DNA can be used directly in a variety of downstream applications, including PCR, RealTime PCR, southern blotting and restriction enzyme digestion. The kit eliminates the need for expensive resin, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yield purified DNA greater than 50 Kb in size. This kit allows for the single or multiple simultaneous processing of samples.
Description
High quality Spin columns, with Proteinase K powder and Poteinase Buffer, Genomic Digestion Buffer, Lysis buffer, wash Buffer 1 & 2, Elution buffer and protocols.
Features
| Application | Isolation of gDNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Automated use | No |
| Sample material | whole Blood, body fluids, buccal swabs |
| Sample amount | < 200 mg |
| Fragment size | 150 bp–approx. 50 kbp |
| Typical yield | 0.1–80 µg (200 µLwhole blood) |
| Theoretical binding capacity | 80 µg |
| Typical purity A260/A280 | 1.7–1.9 |
| Elution volume | 100 µL |
| Preparation time | 20 min/12 preps |
| Typical downstream application | Real-time PCR, Southern blotting |
| Storage temperature | 18−25 °C |
| Shelf life (from production) | 12 Months |
Technical Data
TaqGen® Whole Blood Genomic DNA Purification
Applications
- Life science research
- Livestock breeding
- Pedigree genotyping
- Veterinary pathogen research
- Routine applied testing
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