TaqGen® Tissue Genomic DNA Purification
Taqgen® Tissue Genomic DNA Purification, a spin column based kit, provide an easy, rapid and cost-effective method to purify genomic DNA from various samples such as Food, Feed, GMO Crops, Meat and Meat products, Human or animal tissue, Dried Blood Spot, bacteria, yeast, cultured cells, tissue, buffy coat, lymphocytes, paraffin-embedded tissue and forensic specimens, without phenol/chloroform extraction and ethanol precipitation. The purified DNA can be used directly in a variety of downstream applications, including PCR, Real-Time PCR, southern blotting and restriction enzyme digestion. The kit can process up to 200 mg animal tissue, mouse tail, paraffin-embedded tissue, 5 x 106 cultured cells can be readily processed. This kit allows for the single or multiple simultaneous processing of samples.
Description
High quality Spin columns, with Proteinase K powder and Poteinase Buffer, Genomic Digestion Buffer, Lysis buffer, wash Buffer 1 & 2, Elution buffer and protocols.
Features
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Application Isolation of DNA CE certified No, research use only Technology Silica membrane technology Automated use No Sample material Feed, Food, Forensic, Human or animal tissue Sample amount < 200 mg Fragment size 150 bp–approx. 50 kbp Typical yield 0.1–80 µg (200 mg Tissue) Theoretical binding capacity 80 µg Typical purity A260/A280 1.7–1.9 Elution volume 100 µL Preparation time 30 min/12 preps Typical downstream application Real-time PCR, Southern blotting Storage temperature 18−25 °C Shelf life (from production) 12 Months
Applications
- Life science research
- Livestock breeding
- Pedigree genotyping
- Veterinary pathogen research
- Routine applied testing
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